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 Post subject: Polyploidy
PostPosted: Fri Jul 09, 2010 3:21 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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Just a quick update on my attempts to induce polyploidy. One of the treated bamboos (Pl.viridistriatus 'Chrysophyllus') died. One of the two pots of Phy. aureosulcata died. Ps. viridula is still alive, but has not produced shoots. The surviving Phy. aureosulcata has produced some slowly growing shoots. Ps. japonica has produced some shoots. And recently the two pots Sasaella masamuneana 'Albostriata' have produced a shoot a piece.

I have no idea if I have induced polyploidy in any of these, but one of them does look slightly different.

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 Post subject: Re: Polyploidy
PostPosted: Sat Jul 10, 2010 2:57 pm 
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The treated Ps. japonica has produced a new, larger shoot.

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The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Sat Jul 10, 2010 5:56 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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And there is also a treated A. gigantea that has done nothing.

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The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Sun Jul 11, 2010 1:13 pm 
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Location: Middle Tennessee (Murfreesboro) USDA Zone 6b/7a Record low Jan 1966 -14*F Frost free April 21-Oct.21 Location Details
Hi Dan-

Perhaps you could outline your process for us (at you convenience).

David

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 Post subject: Re: Polyploidy
PostPosted: Mon Jul 12, 2010 3:35 am 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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I'm still experimenting to work out the details of the process, but it seems to work out to soak the rhizomes for 24 hours in a solution of one part surflan to 10,000 parts water heated to 80°F. After 24 hours of soaking I thoroughly rinse the rhisomes and pot the bamboo. I figure the warm solution would get the buds on the rhizomes to growing. I have left old culms attached to the rhizomes to provide energy for growth.

Now the question is, does it work?

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The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Mon Jul 12, 2010 2:43 pm 
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Location: St. Louis area Location Details
The real question is "what do you hope happens?" :)

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My blog: It's not work, it's gardening!


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 Post subject: Re: Polyploidy
PostPosted: Mon Jul 12, 2010 3:25 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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Alan_L wrote:
The real question is "what do you hope happens?" :)


Some type of change in the plant other than it dying. My main goal is to see if I can induce polyploidy in some of the species that are known to be hybrids. But if the treatment causes some other stable change in the bamboo, such as coloration, leaf shape, culm shape, etc., I would be willing to accpet that as a successful result. :)

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The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Sun Jul 25, 2010 1:04 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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The treated A. gigantea has decided to start leafing out. Not dead yet!

Since these are culms I left attached to the rhizome to provide energy for (hopefully) the growth of the polyploid rhizome buds.

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Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Wed Aug 25, 2010 3:57 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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The remaining Phy. aureosulcata is just about dead.
Ps. viridula is still alive, but has not produced shoots.
Ps. japonica is looking well.
The two pots Sasaella masamuneana 'Albostriata' have continued to produce shoots.
A. gigantea has produced a small shoot.

The wait continues...

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Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Sat Sep 04, 2010 6:29 pm 
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Location: Sydenham, Ontario, Canada
Do you have access to a microscope and chromsome stain? I can walk you through the fixing process if you do, at least then you could have a rough idea of whether it's working... Might be interesting to have a look and see if any of the boo's have, in fact, become polyploid mosaics... I guess the goal will be to isolate the polyploid plants from the mosaic clump?

I forgot to mention this before, but, when I induced polyploidy (by mistake) in some plants I regenerated via leaf cutting (in culture), there was an obvious drop in pollen fertility from 'normal' (c. 80%) to about 20-40%... And also the viable pollen becomes much larger (1.5x the size of 'normal' pollen). Just another factor that I though you should know about. It shouldn't affect your attempts at cross pollinating since there should still plenty of viable pollen!

Keep us updated!


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 Post subject: Re: Polyploidy
PostPosted: Thu Jun 23, 2011 7:30 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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Update:
Ps. viridula is still alive and has produced one shoot.
Ps. japonica is looking well.
The two pots Sasaella masamuneana 'Albostriata' died.
A. gigantea has not produced any more shoots, but is still alive.

No access to a microscope and chromsome stain that I know of.

_________________
Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Thu Jun 23, 2011 10:41 pm 
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Location: Bradenton, Florida
Do you have pictures that you would care to share....

It is always easiest when pics are involved...LOL

Jan


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 Post subject: Re: Polyploidy
PostPosted: Wed Sep 21, 2011 1:49 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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Update:
Ps. viridula is still alive and is producing shoots.
Ps. japonica is looking well.
A. gigantea has not produced any more shoots, but is still alive.

_________________
Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Wed Feb 08, 2012 9:33 pm 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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Update:
Ps. viridula is looking well and is producing shoots.
Ps. japonica is looking well and has probably produced shoots.
A. gigantea has not produced any more shoots, but is still alive. I have noticed a few new leaves and there is something about the base of the culms. I'm not sure, but the look a bit swollen to me.

_________________
Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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 Post subject: Re: Polyploidy
PostPosted: Fri Apr 13, 2012 1:54 am 
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Location: Zone 5b/6a Bloomington, INElevation: 770-790 feet Location Details
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A. gigantea has produced a tiny shoot.

_________________
Southern Indiana.
My Bamboo List.

The legal issues that will arise when the undead walk the earth are legion, and addressing them all is well beyond what could reasonably be accomplished in this brief Essay. Indeed, a complete treatment of the tax issues alone would require several volumes.


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