Polyploidy

foxd · **Posted:** Fri Jul 09, 2010 3:21 pm

Just a quick update on my attempts to induce polyploidy. One of the treated bamboos (Pl.viridistriatus 'Chrysophyllus') died. One of the two pots of Phy. aureosulcata died. Ps. viridula is still alive, but has not produced shoots. The surviving Phy. aureosulcata has produced some slowly growing shoots. Ps. japonica has produced some shoots. And recently the two pots Sasaella masamuneana 'Albostriata' have produced a shoot a piece.

I have no idea if I have induced polyploidy in any of these, but one of them does look slightly different.

foxd · **Posted:** Sat Jul 10, 2010 2:57 pm

The treated Ps. japonica has produced a new, larger shoot.

foxd · **Posted:** Sat Jul 10, 2010 5:56 pm

And there is also a treated A. gigantea that has done nothing.

David · **Posted:** Sun Jul 11, 2010 1:13 pm

Hi Dan-

Perhaps you could outline your process for us (at you convenience).

David

foxd · **Posted:** Mon Jul 12, 2010 3:35 am

I'm still experimenting to work out the details of the process, but it seems to work out to soak the rhizomes for 24 hours in a solution of one part surflan to 10,000 parts water heated to 80°F. After 24 hours of soaking I thoroughly rinse the rhisomes and pot the bamboo. I figure the warm solution would get the buds on the rhizomes to growing. I have left old culms attached to the rhizomes to provide energy for growth.

Now the question is, does it work?

Alan_L · **Posted:** Mon Jul 12, 2010 2:43 pm

The real question is "what do you hope happens?"

foxd · **Posted:** Mon Jul 12, 2010 3:25 pm

Alan_L wrote:

The real question is "what do you hope happens?"

Some type of change in the plant other than it dying. My main goal is to see if I can induce polyploidy in some of the species that are known to be hybrids. But if the treatment causes some other stable change in the bamboo, such as coloration, leaf shape, culm shape, etc., I would be willing to accpet that as a successful result.

foxd · **Posted:** Sun Jul 25, 2010 1:04 pm

The treated A. gigantea has decided to start leafing out. Not dead yet!

Since these are culms I left attached to the rhizome to provide energy for (hopefully) the growth of the polyploid rhizome buds.

foxd · **Posted:** Wed Aug 25, 2010 3:57 pm

The remaining Phy. aureosulcata is just about dead.
Ps. viridula is still alive, but has not produced shoots.
Ps. japonica is looking well.
The two pots Sasaella masamuneana 'Albostriata' have continued to produce shoots.
A. gigantea has produced a small shoot.

The wait continues...

Paul Ont · **Posted:** Sat Sep 04, 2010 6:29 pm

Do you have access to a microscope and chromsome stain? I can walk you through the fixing process if you do, at least then you could have a rough idea of whether it's working... Might be interesting to have a look and see if any of the boo's have, in fact, become polyploid mosaics... I guess the goal will be to isolate the polyploid plants from the mosaic clump?

I forgot to mention this before, but, when I induced polyploidy (by mistake) in some plants I regenerated via leaf cutting (in culture), there was an obvious drop in pollen fertility from 'normal' (c. 80%) to about 20-40%... And also the viable pollen becomes much larger (1.5x the size of 'normal' pollen). Just another factor that I though you should know about. It shouldn't affect your attempts at cross pollinating since there should still plenty of viable pollen!

Keep us updated!

foxd · **Posted:** Thu Jun 23, 2011 7:30 pm

Update:
Ps. viridula is still alive and has produced one shoot.
Ps. japonica is looking well.
The two pots Sasaella masamuneana 'Albostriata' died.
A. gigantea has not produced any more shoots, but is still alive.

No access to a microscope and chromsome stain that I know of.

meadowyck · **Posted:** Thu Jun 23, 2011 10:41 pm

Do you have pictures that you would care to share....

It is always easiest when pics are involved...LOL

Jan

foxd · **Posted:** Wed Sep 21, 2011 1:49 pm

Update:
Ps. viridula is still alive and is producing shoots.
Ps. japonica is looking well.
A. gigantea has not produced any more shoots, but is still alive.

foxd · **Posted:** Wed Feb 08, 2012 9:33 pm

Update:
Ps. viridula is looking well and is producing shoots.
Ps. japonica is looking well and has probably produced shoots.
A. gigantea has not produced any more shoots, but is still alive. I have noticed a few new leaves and there is something about the base of the culms. I'm not sure, but the look a bit swollen to me.

foxd · **Posted:** Fri Apr 13, 2012 1:54 am

A. gigantea has produced a tiny shoot.

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Polyploidy

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